3D Microscopy in Extreme Conditions: In-Flow or at High Density
Y. Shechtman, E. Nehme, B. Ferdman, O. Adir, R. Gordon-Soffer, R. Orange, T. Naor, Y. Shalev-Ezra, S. Goldberg, O. Alalouf, D. Freedman, A. Schroeder, T. Michaeli, and L. Weiss
SPIE Photonics West BiOS, 2021
Attaining three-dimensional data at high throughput is a grand challenge in microscopy. I will discuss two recent contributions to 3D microscopy utilizing point-spread-function (PSF) engineering: 1.a new method that extends the capabilities of flow-based imaging to 3D co-localization microscopy, and 2. efficient and fast localization of dense molecules in volumetric single-molecule-localization microscopy (SMLM) using deep learning, termed DeepSTORM3D. Together, these methods enable the study of sub-cellular biological processes at unprecedented timescales and throughputs.